Regulatory

Part:BBa_K2391001:Design

Designed by: Texia Loh   Group: iGEM17_Washington   (2017-10-27)


Zinc-responsive Activator Protein (ZAP1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It should be noted that in a zinc limited environment, ZAP1 is active. Conversely ZAP1 is repressed when zinc is repleted.

We considered if there were any additional parts we would need to add to the plasmid and what protein to use for characterization.

Source

In the process of creating our biobrick, we checked that the ZAP1 sequence did not contain any enzyme cut sites similar to those of the prefix and suffix, required by the iGEM Biobricks Standard Assembly protocol. Through IDT we were able to order a gBlock with our sequence of interest with the prefix and suffix attached.

References

Frey, Avery G. et al. “Zinc-Regulated DNA Binding of the Yeast Zap1 Zinc-Responsive Activator.” Ed. Juan Mata. PLoS ONE 6.7 (2011): e22535. PMC. Web. 2 Nov. 2017.