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Part:BBa_K2391001:Design
Zinc-responsive Activator Protein (ZAP1)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
It should be noted that in a zinc limited environment, ZAP1 is active. Conversely ZAP1 is repressed when zinc is repleted.
We considered if there were any additional parts we would need to add to the plasmid and what protein to use for characterization.
Source
In the process of creating our biobrick, we checked that the ZAP1 sequence did not contain any enzyme cut sites similar to those of the prefix and suffix, required by the iGEM Biobricks Standard Assembly protocol. Through IDT we were able to order a gBlock with our sequence of interest with the prefix and suffix attached.
References
Frey, Avery G. et al. “Zinc-Regulated DNA Binding of the Yeast Zap1 Zinc-Responsive Activator.” Ed. Juan Mata. PLoS ONE 6.7 (2011): e22535. PMC. Web. 2 Nov. 2017.